Colbert jests that he may lose his show under Trump, lamenting the end of the Biden administration.

On Thursday, Stephen Colbert expressed his sadness over the conclusion of President Biden's term in office, marking the end of his time hosting "The Late Show."

Colbert joked that he may not be on the air anymore during President-elect Donald Trump's second term, which will begin on Monday, Jan. 20, marking the end of an era.

The anti-Trump comedian thanked his audience for their support and informed them that the next time they see him, Donald Trump will be president.

The late-night host stated that the broadcast would be tinged with sadness due to Biden's departure from the White House.

"Tonight, we'll enjoy a little sweet and sour, a little semi-sweet chocolate chip. This is the end of an era, as it marks the last show of the Biden Administration."

The in-house audience responded with a sigh of disappointment.

The host stated that the show's crew will approach the next four years "one day at a time."

Throughout Trump's political career, Colbert's show has provided strong opposition to the president through humor.

During Trump's second presidential campaign, Colbert criticized him 40 times more than Vice President Harris, according to a Media Research Center study.

During his presidential farewell address on Wednesday, Biden was mocked by Colbert for a "meandering metaphor" he used.

The president's speech was about the Statue of Liberty, which is not stationary but moves to withstand storms and sway a few inches without falling.

When the camera returned to Colbert, he was wearing aviator sunglasses and doing a Biden impression. 'What I’m saying is, run! The Statue of Liberty is alive, everybody! Get out of there! No, no, It’s alive! I saw it in a documentary – ‘Ghostbusters 2.’ You got to spray the insides with that angry goo. Don’t cross the streams.'

"Joe Biden out, everybody!" Colbert said, ending the bit.

In July, after Biden's exit from the 2024 presidential race, Colbert hosted a glitzy fundraiser for the president's re-election campaign.